N K L designed and performed the CRISPR
N.K.L. designed and performed the CRISPR screen and validation experi-ments, isolated tumor Nutlin-3 for ChIP-seq, prepared samples for RNA-seq, per-formed all functional experiments related to RORg inhibition in vitro and in vivo, and performed histologic analysis and live imaging experiments. L.P.F. carried out CRISPR screen validation and related bioinformatic analysis, in vivo RORg target analysis, protein expression, cytokine analysis, RNA-seq library prepa-ration and helped with manuscript and Þgure preparation. N. Rajbhandari analyzed Rorc / KPf/fC mice and helped with in vivo drug studies and CRISPR screen validation; R.F., T.G., and L.A.E. provided experimental help; P.S., N.E.L., M.H., K.M.F., R.S., and S.B.R. performed bioinformatics analysis related to RNA-seq and CRISPR screen; P.N. and H.H. carried out the single-cell RNA-seq and W.L. and M.H. performed related computational analysis; A.D. and A.J.D. performed ChIP-seq; K.G., N. Robertson, and P.D.A. performed all ChIP-seq analysis; and for tissue microarray (TMA) anal-ysis, C.M.S. and M.W. analyzed and interpreted TMA staining, C.M.S. created Þgures and wrote the report, I.Z. performed statistical analysis, M.E. created Scorenado and provided technical advice, J.A.G. performed TMA immunohis-tochemistry (IHC) staining, and E.K. created the TMAs and collected clinical data. T.I., D.V.H., A.M.L., and P.D.A. provided experimental and/or computa-tional advice and comments on the manuscript. N.K.L., L.P.F., and M.K. helped write the paper. T.R. conceived of the project, planned and guided the research, and wrote the paper.
DECLARATION OF INTERESTS
This work includes data Þled as a provisional application SD2019-243. T.R. is a consultant for Orphagen Pharmaceuticals, and D.V.H. serves on the ScientiÞc Advisory Board of Five Prime and is a consultant for Celgene.
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KEY RESOURCES TABLE
REAGENT or RESOURCE
Anti Mouse Pan-Keratin
Anti mouse CD31-PE
Anti mouse CD4-PaciÞc blue